Professor Reinhard Büttner: Histopathology (Institute for Pathology)

Histopathology is the main connecting cross-disciplinary subject in medicine, and enables the
direct visualization of tissue changes. This technique has the unique capacity to analyze a marker
at the single cell/tissue level while preserving the morphological context. Formalin-fixed and
paraffin-embedded (FFPE) human as well as mouse material will be used for a morphologybased
description of the different tissue components using histochemical, immunohistochemical,
as well as in situ, technologies (RNAscope).
Immunohistochemistry (IHC) allows the detection of cell-associated or non-cell fixed proteins,
modern in situ hybridization (ISH) technologies enable the reliable and specific detection of
mRNA, miRNA, and lncRNA within the tissue environment context. Dual ISH and IHC stainings
will be used for the identification of cytokines and their cellular origin. Furthermore, the
Nanostring technology enables the expression profiling of more than 500 inflammation- and
immunology-related genes on human or mouse FFPE material.
1) Histopathology: Standard tissue preparation on FFPE material or frozen material using
different histochemical stainings.
2) Immunohistochemistry: Standard detection of different proteins on-slides in the preserved
tissue context.
3) RNAScope: Specific detection of mRNA, miRNA, and lncRNA within the tissue environment,
4) Nanostring platform: Inflammation- and immunology-related expression profiling of more
than 500 genes on human or mouse FFPE material.
Internal resources: Immunohistochemical detection platforms (Bond MAX by Leica, Benchmark
by Ventana, Dako Stainer); RNAscope platform including dual ISH-IHC staining (Ventana
Discovery XT); Nanostring platform.